Leo: Straight and demisexual
Raph: Straight and demiaromatic
Donnie: Bisexual and either demisexual or asexual
Mikey: Either gay or pansexual and demisexual
April: Bisexual and either demiaromatic or demisexual
Casey: Bisexual and demiaromatic
Splinter: Straight and demisexual
Shredder: Straight
Baxter Stockman: Idk
Bebop: Gay
Rocksteady: Gay
Dr Rockwell: Idk but is asexual
Fishface: Either bisexual or gay
Fugitoid: Asexual and demiaromatic
Karai: Either lesbian or bisexual and demiaromatic
Kirby: Straight
Kraang Subprime/Irma: Asexual and aromatic
Leatherhead: Either gay or pansexual and asexual
Lord Dregg: Idk
Mona Lisa: Straight, demisexual and demiaromatic
Mondo Gecko: Bisexual
Mr. Murakami: Idk but is asexual
Muckman: Idk
Napoleon: Gay
Newtrailzer: Idk
Pigeon Pete: Idk
Rahzar: Either bisexual or gay
Renet: Pansexual and asexual
Sal Commander: Idk
Tiger claw: Idk but is into boys
Alopex: Idk
Slash: Bisexual, demisexual and demiaromatic
Shinigami: Lesbian and demiaromatic