I'm guessing characters that I have already tried to guess their sexualities!

Class 1-A!
Izuku: Male
Katsuki: Transgender (ftm)
Shoto: Transgender (ftm)
Eijiro: Male
Denki: Transgender (ftm)
Ochaco: Transgender (mtf)
Tenya: Male
Mina: Female
Momo: Female
Kyoka: Female
Mashiaro: Male
Toru: Female
Minoru: Either bigender or gender fluid (Pronounces if bigender are either he/she or he/they!)
Yuga: Either male or non binary
Tsuyu: Female
Hanta: Non binary
Fumikage: Bigender (Pronounces are he/they!)
Mezo: Agender
Rikido: Male
Koji: Transgender (ftm)

Gravity falls!
Dipper: Transgender (ftm)
Mabel: Female
Stan: Male
Soos: Either male or non binary
Old man McGucket: Either male or bigender (Pronounces if bigender are he/they!)
Bill Cipher: Bigender (Pronounces are he/they!)
Wendy: Female
Ford: Either male or non binary
Candy: Female
Grenda: Transgender (mtf)
Deputy Durland: Male
Sheriff Blubs: Male
Gideon: Male
Pacifica: Female
Robbie: Transgender (ftm)

Tmnt 2012!
Leo: Male
Raph: Male
Donnie: Transgender (ftm)
Mikey: Male
April: Either transgender or bigender (Mtf and pronounces if bigender are she/they!)
Casey: Transgender (ftm)
Splinter: Male
Shredder: Male
Baxter Stockman: Male
Bebop: Male
Rocksteady: Either male or non binary
Dr Rockwell: Male
Fishface: Male
Fugitoid: Bigender (Pronounces are he/they!)
Karai: Female
Kirby: Male
Kraang Subprime/Irma: Bigender (Pronounces are he/her!)
Leatherhead: Either male or non binary
Lord Dregg: Agender
Mona Lisa: Female
Mondo Gecko: Non binary
Mr. Murakami: Male
Muckman: Either male or non binary
Napoleon: Gender fluid
Newtrailzer: Agender
Pigeon Pete: Male
Rahzar: Male
Renet: Female
Sal Commander: Non binary
Tiger claw: Male
Alopex: Female
Slash: Male
Shinigami: Transgender (mtf)

Total drama!
Amy: Female
Chris: Male
Sugar: Female
Max: Transgender (ftm)
Leonard: Male
Blainley: Female
Chet: Male
Lorenzo: Transgender (ftm)
Staci: Female
Topher: Transgender (ftm)
Rodney: Male
Stephanie: Female
Courtney: Female
Dave: Male
Tammy: Female
Taylor: Female
Jo: Either female or non binary
Pete: Male
Gerry: Male
Justin: Male
Josee: Female
Mickey: Transgender (ftm)
Jay: Transgender (ftm)
Anne Maria: Female
Eva: Either agender or non binary
Kelly: Female
Ezekiel: Non binary
Lighting: Male
Beardo: Agender
Laurie: Female
Miles: Female
Zoey: Female
Scarlett: Female
Ryan: Male
Mary: Female
Ellody: Female
Beth: Female
Katie: Female
Sadie: Female
Jacques: Male
Heather: Female
Dakota: Either female or bigender (Pronounces are she/they!)
Spud: Non binary
Rock: Transgender (ftm)
Duncan: Male
Sky: Female
Devin: Male
Cody: Male
Cameron: Transgender (ftm)
Sam: Male
Jen: Female
Tom: Male
Scott: Male
Harold: Male
Sierra: Female
Mike: Gender fluid
Don: Male
Crimson: Female
Ennui: Male
Dwayne: Male
Chef: Male
B: Non binary
Tyler: Male
Leshawna: Female
Brody: Male
Geoff: Male
Junior: Transgender (ftm)
Sammy: Transgender (mtf)
Alejandro: Male
Owen: Male
Noah: Transgender (ftm)
Izzy: Non binary
Sanders: Female
MacArthur: Female
Trent: Male
Emma: Female
Kitty: Female
DJ: Male
Gwen: Either gender fluid or non binary
Lindsay: Female
Dawn: Either gender fluid or non binary
Bridgette: Female
Shawn: Male
Brick: Transgender (ftm)
Ella: Female
Jasmine: Female

Harry Potter!
Harry: Transgender (ftm)
Ron: Male
Hermione: Female
Ginny: Female
Neville: Transgender (ftm)
Luna: Transgender (mtf)
George: Either male or non binary
Fred: Either male or agender
Lavender: Female
Sirius: Male
Remus: Male
Cho: Either female or transgender (mtf)
Draco: Transgender (ftm)
Pansy: Female
Vincent: Male
Gregory: Male
Brase: Male
Severus: Male
Voldemort/Tom: Agender
Lucius: Male
Dumbledore: Male
Colin: Male
Cedric: Male

Steven Universe!
Steven: Male
Garnet: Bigender (Pronounces are she/they!)
Amethyst: Bigender (Pronounces are she/they!)
Pearl: Bigender (Pronounces are she/they!)
Lars: Male
Sadie: Female
Greg: Male
Jamie: Male
Buck: Non binary
Sour cream: Male
Jenny: Female
Ronaldo: Non binary
Peridot: Gender fluid
Lapis: Bigender (Pronounces are she/they!)
Jasper: Agender
Onion: Agender
Connie: Female
White diamond: Bigender (Pronounces are she/they!)
Blue diamond: Bigender (Pronounces are she/they!)
Yellow diamond: Bigender (Pronounces are she/they!)
Pink diamond/Rose quartz: Bigender (Pronounces are she/they!)
Eyeball: Agender
Aquamarine: Bigender (Pronounces are she/they!)
Spinel: Either gender fluid or bigender (Pronounces if bigender are she/they!)

Persona!
Minato: Male
Minako: Female
Yukari: Female
Junpei: Male
Mitsuru: Transgender (mtf)
Akihiko: Male
Fuuka: Non binary
Aigis: Bigender (Pronounces are she/they!)
Ken: Male
Shinjiro: Transgender (ftm)
Metis: Non binary
Ryoji/Pharos: Agender
Yu: Male
Yosuke: Transgender (ftm)
Chie: Female
Yukiko: Female
Kanji: Male
Rise: Female
Teddie: Male
Naoto: Either gender fluid or non binary or transgender (ftm)
Adachi: Male
Taro: Male
Akira: Male
Morgana: Male
Ryuji: Male
Ann: Female
Yusuke: Non binary
Makoto: Female
Futaba: Gender fluid
Haru: Female
Akechi: Male
Sumire: Female
Caroline/Justine/Lavenza: Female
Margaret: Female
Marie: Bigender (Pronounces are she/they!)
Theodore: Transgender (ftm)
Elizabeth: Either gender fluid or non binary
Igor: Either male or agender
Toranosuke: Male
Shinya: Innersex (Pronounces are they/them!)
Munehisa: Either male or non binary
Yuuki: Transgender (ftm)
Sojiro: Male
Ichiko: Female
Hifumi: Either female or non binary
Chihaya: Gender fluid
Sadayo: Female
Tae: Bigender (Pronounces are she/they!)
Sae: Female
Takuto: Male
Kobayakawa: Male
Hiruta: Male
Inui: Male
Ushimaru: Male
Chouno: Transgender (mtf)
Usami: Either female or non binary
Shiho: Female
Suguru: Male
Ichiryusai: Male
Junya: Male
Kunikazu: Male
Masayoshi: Male
SIU director: Male
Wakaba: Female
Jose: Agender
Natsuhiko: Male
Mika: Female
Sugimura: Male
Shinichi: Male
Hiraguchi: Female
Kasumi: Female
Shibusawa: Male
Rumi: Female
Lala: Transgender (mtf)
Ryotaro: Male
Nanako: Female
Mitsuo: Non binary
Sayoko: Female
Shu: Transgender (ftm)
Eri: Female
Yuuta: Male
Kinshiro: Male
Noriko: Female
Saki: Either female or non binary
Hanako: Female
Ai: Female
Kou: Male
Daisuke: Male
Ayane: Bigender (Pronounces are she/they!)
Yumi: Either female or transgender (mtf)
Naoki: Male
Hisano: Female
Mayumi: Female
Misuzu: Female
Shiroku: Female
Daidara: Male
Shuji: Male
Isako: Female
Edogawa: Non binary
Emiri: Female
Ounishi: Female
Ekoda: Male
Terauchi: Female
Miyahara: Gender fluid
Ono: Male
Kenji: Male
Hidetoshi: Male
Nozomi: Non binary
Chihiro: Female
Andre: Gender fluid in
Keisuke: Transgender (ftm)
Kazushi: Male
Yuko: Female
Rio: Female
Saori: Non binary
Natsuki: Transgender (mtf)
Kiyoshi: Male
Takaya: Agender
Jin: Gender fluid
Chidori: Female
Kurosawa: Male
Maiko: Female
Bunkichi: Male
Mitsuko: Female
Akinari: Non binary
Mamoru: Male
Mutatsu: Male
Tanaka: Male

Danganronpa!
Makoto: Male
Sayaka: Female
Leon: Male
Hifumi: Male
Toko: Female
Kiyotaka: Transgender (ftm)
Aoi: Female
Chihiro: Gender fluid
Kyoko: Transgender (mtf)
Junko: Female
Mondo: Either male or non binary
Celeste: Female
Yasuhiro: Either male or non binary
Byakuya: Male
Sakura: Transgender (mtf)
Hajime: Male
Nagito: Either male or transgender (ftm)
Hiyoko: Female
Akane: Female
Kazuichi: Either male or non binary
Gundham: Bigender (Pronounces are he/they!)
Mikan: Female
Ibuki: Transgender (mtf)
Nekomaru: Male
Fuyuhiko: Transgender (ftm)
Mahiru: Female
Peko: Transgender (mtf)
Chiaki: Female
Teruteru: Male
Sonia: Female
Shuichi: Transgender (ftm)
Kaede: Transgender (mtf)
Tsumugi: Female
Rantaro: Transgender (ftm)
Himiko: Female
Tenko: Female
Miu: Female
Keebo: Agender
Kokichi: Either male or non binary
Ryoma: Non binary
Maki: Female
Korekiyo: Non binary
Kaito: Male
Kirumi: Either female or non binary
Gonta: Either male or non binary
Angie: Female
Komaru: Female
Nagisa: Innersex (Pronounces are he/him!)
Jataro: Agender
Kotoko: Female
Monaca: Female
Masaru: Male
Mukuro: Bigender (Pronounces are she/they!)

Fnaf!
William: Male
Clara: Female
Michael: Male
Elizabeth: Female
Chris: Male
Nightmare: Male
Nightmare golden Freddy: Male
Herny: Male
Terrance: Transgender (ftm)
Charlie: Female
Glitchtrap: Male
Vanny: Female
Ennard: Male
Freddy: Male
Bonnie: Non binary
Chica: Female
Foxy: Male
Golden Freddy: Bigender (Pronounces are he/her!)
Toy Freddy: Male
Toy Bonnie: Male
Toy Chica: Female
Mangle: Agender
Balloon boy: Male
JJ: Female
DeeDee: Either gender fluid or non binary
Minireenas: Agender
Funtime Foxy: Either female or non binary
Lolbit: Either gender fluid or non binary
Funtime Freddy: Male
Yenndo: Non binary
Bon-Bon: Bigender (Pronounces are he/they!)
Bonnet: Bigender (Pronounces are sshe/they!)
Bibybab: Agender

Marvel!
Iron man: Transgender (ftm)
Thor: Male
Hulk: Bigender (Pronounces is he/they!)
Wasp: Female
Ant-man: Male
Captain America: Male
Hawkeye: Male
Quicksilver: Male
Scarlet witch: Female
Black Panther: Male
Vision: Bigender (Pronounces are he/they!)
Captain Marvel: Female
Falcon: Male
War machine: Male
Loki: Non binary
Nick Fury: Male
Spider-man: Transgender (ftm)
Black widow: Transgender (mtf)
Dr Strange: Either male or non binary
Bucky: Male
Drax: Male
Groot: Agender
Gamora: Female
Rocket: Non binary
Mantis: Bigender (Pronounces are she/they!)
Nebula: Bigender (Pronounces are she/they!)
Star lord: Male
Shuri: Transgender (mtf)
Deadpool: Male