Cau 13: Trinh bay dn va co che cua cac htuong: bien nap, tai nap, tiep hop

*Bien nap

-DN: bien nap la su van chuyen 1 doan AND cua vk cho nap vao vk nhan

-Dkien:+Vk cho fai bi fa vo (ly jai)

+NST cua no dc jai fong va bi cat thanh nhung doan AND nho

+Vk nhan fai o trang thai sinh ly dac biet, cho fep nhung manh AND xam nhap vao tbao

-2 gdoan xay ra (.) qtrinh bien nap

+Nhan manh AND

+Tich hop manh AND da nhan vao NST qua tai to hop kinh dien

H.tuong bien nap thay o lien cau nao mo cau...

Ki thuat bien nap dc ap dung trong cong nghe sinh hoc la bien nap gen tong hop Insulin vao tbao E.Coli or nam men de san xuat Insulin. *)Tiep hop

-DN tiep hop la su van chuyen chat lieu di truyen tu vk duc sang vk cai khi 2 vk tiep xuc voi nhau

-Dkien: 1 vk fai co yeu to joi tinh F, tuc la co Pili joi tinh lam cau giao foi. Nhung vk co yeu to F goi la vk duc F+, vk k co yeu to F goi la vk cai F-

-Yeu to F co the ton tai o 3 trang thai:

+F+: yeu to F nam trong nguyen tuong

+Hfr: yeu to F tich hop vao NST

+F': sau khi yeu to F tich hop vao NST lai roi ra, nam tu do (.) nguyen tuong nhung co mang theo 1 doan AND cua NST. Tiep hop thuong xay ra jua nhung vk cung loai nhung cung co the xay ra jua nhung vk khac loai nhu E.Coli voi Salmonella or Shigenlla nhung tan so tai to hop thap

-3 gdoan: +Tiep hop 2 tbao qua cau jao foi (pili joi tinh)

+Chuyen gen

+Tich hop doan gen chuyen vao NST cua vk nhan qua tai to hop kinh dien

*Tai nap

-Dn: tai nap la su van chuyen chat lieu di truyen tu vk cho nap vao vk nhan nho phage

-Cac loai tai nap

+Tai nap han che va dac hieu: 1 phage nhat dinh chi mang dc 1 gen nhat dinh tu vk cho sang nap vao vk nhan. +) Tai nap chung: phage co the mang bat ki 1 loai gen nao cua vk cho sang nap vao vk nhan

., Tai nap chung hoan chinh: doan gen mang sang dc tich hop vao NST cua vk nhan qua tai to hop do do dc nhan len cung NST va co mat o cac the he sau (it gap)

.,Tai nap chung k hoan chinh: doan gen mang sang k dc nap vao NST cua vk nhan ( k dc nhan len ma

chi nam lai o 1 tbao con khi vk fan chia. Dac tinh cua gen mang sang van dc bieu hien sang kieu hinh song chi o 1 tbao duy nhat hay g